Isoflurane differentially affects neurogenesis and long-term neurocognitive function in 60-day-old and 7-day-old rats.

نویسندگان

  • Greg Stratmann
  • Jeffrey W Sall
  • Laura D V May
  • Joseph S Bell
  • Kathy R Magnusson
  • Vinuta Rau
  • Kavel H Visrodia
  • Rehan S Alvi
  • Ban Ku
  • Michael T Lee
  • Ran Dai
چکیده

BACKGROUND Anesthetic agents cause cell death in the developing rodent brain and long-term, mostly hippocampal-dependent, neurocognitive dysfunction. However, a causal link between these findings has not been shown. Postnatal hippocampal neurogenesis affects hippocampal function into adulthood; therefore, the authors tested the hypothesis that isoflurane affects long-term neurocognitive function via an effect on dentate gyrus neurogenesis. METHODS The S-phase marker 5-bromodeoxyuridine was administered at various times before, during, and after 4 h of isoflurane given to postnatal day (P)60 and P7 rats to assess dentate gyrus progenitor proliferation, early neuronal lineage selection, and long-term survival of new granule cell neurons. Fear conditioning and spatial reference memory was tested at various intervals from 2 weeks until 8 months after anesthesia. RESULTS In P60 rats, isoflurane increased early neuronal differentiation as assessed by BrdU/NeuroD costaining, decreased progenitor proliferation for 1 day, and subsequently increased progenitor proliferation 5-10 days after anesthesia. In P7 rats, isoflurane did not induce neuronal lineage selection but decreased progenitor proliferation until at least 5 days after anesthesia. Isoflurane improved spatial reference memory of P60 rats long-term, but it caused a delayed-onset, progressive, persistent hippocampal deficit in P7 rats in fear conditioning and spatial reference memory tasks. CONCLUSION The authors conclude that isoflurane differentially affects both neurogenesis and long-term neurocognitive function in P60 and P7 rats. Neurogenesis might mediate the long-term neurocognitive outcome after isoflurane at different ages.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isoflurane does not affect brain cell death, hippocampal neurogenesis, or long-term neurocognitive outcome in aged rats.

BACKGROUND Roughly, 10% of elderly patients develop postoperative cognitive dysfunction. General anesthesia impairs spatial memory in aged rats, but the mechanism is not known. Hippocampal neurogenesis affects spatial learning and memory in rats, and isoflurane affects neurogenesis in neonatal and young adult rats. We tested the hypothesis that isoflurane impairs neurogenesis and hippocampal fu...

متن کامل

Distinct long-term neurocognitive outcomes after equipotent sevoflurane or isoflurane anaesthesia in immature rats.

BACKGROUND Many anaesthetics when given to young animals cause cell death and learning deficits that persist until much later in life. Recent attempts to compare the relative safety or toxicity between different agents have not adequately controlled for the relative dose of anaesthetic given, thereby making direct comparisons difficult. METHODS Isoflurane or sevoflurane were given at 1 minimu...

متن کامل

اثر تمرین هوازی شنا و مصرف عصارۀ سیر بر سطوح برخی از فاکتور‌های رشد مداخله‌گر در آنژیوژنز و نروژنز بافت مغز در موش‌های صحرایی پیر

Background and Objective: Angiogenesis and Neurogenesis declines with aging whereas regular exercise and antioxidant supplements are associated with various physiological changes in the brain. Therefore, the aim of this study was to assess the effect of swimming exercise and garlic extract on the some of growth factors involved in angiogenesis and neurogenesis in the brain tissue of old rats. ...

متن کامل

Isoflurane Exposure did not Adversely Affect Recognition Memory or Decrease Hippocampal Brain Derived Neurotrophic Factor Expression in the 17 Day Old Rat Pup

A previous study showed that hippocampal BDNF mRNA decreased in 17 day old (D17) rats, relative to agematched naïve rats, at day 1, 3, 7 and 14 after sham surgery in a traumatic brain injury model. The anesthetic isoflurane activates GABA and inhibits NMDA receptor currents, both of which are known to decrease Brain-Derived Neurotrophic Factor (BDNF) mRNA in rat hippocampi. Hippocampal BDNF is ...

متن کامل

Dexmedetomidine attenuates isoflurane-induced neurocognitive impairment in neonatal rats.

BACKGROUND Neuroapoptosis is induced by the administration of anesthetic agents to the young. As alpha2 adrenoceptor signaling plays a trophic role during development and is neuroprotective in several settings of neuronal injury, the authors investigated whether dexmedetomidine could provide functional protection against isoflurane-induced injury. METHODS Isoflurane-induced injury was provoke...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Anesthesiology

دوره 110 4  شماره 

صفحات  -

تاریخ انتشار 2009